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What is Difference between SEM, TEM, FESEM?

Tingting Z

Mar 4, 2023

In the field of materials science, it is crucial to understand the microstructural and morphological features of materials. Microscopy has played a significant role in this regard, and with the advancement of technology, various types of microscopes have been developed. Among them, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and field-emission scanning electron microscopy (FESEM) are the most widely used techniques. Although these techniques share similarities, they differ in their principles of operation, resolution, and sample preparation requirements. This paper aims to provide a comprehensive overview of these techniques, their principles, and their applications.

What is Difference between SEM, TEM, FESEM?

 

In the field of materials science, it is crucial to understand the microstructural and morphological features of materials. Microscopy has played a significant role in this regard, and with the advancement of technology, various types of microscopes have been developed. Among them, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and field-emission scanning electron microscopy (FESEM) are the most widely used techniques. Although these techniques share similarities, they differ in their principles of operation, resolution, and sample preparation requirements. This paper aims to provide a comprehensive overview of these techniques, their principles, and their applications.

 

Scanning Electron Microscopy (SEM)

Scanning electron microscopy (SEM) is a widely used technique for imaging the surface of materials. The SEM works by scanning a focused electron beam over the surface of the sample, and detecting the secondary electrons emitted from the sample. The secondary electrons are detected by a detector, which converts the signal into an image. SEM is a non-destructive imaging technique, and it can provide high-resolution images of the surface of a wide range of materials, including metals, ceramics, polymers, and biological materials.

SEM operates under high vacuum conditions, and the samples need to be conductive or coated with a conductive material, such as gold or carbon. The electron beam used in SEM has a high energy, typically between 5 and 30 keV, which allows for deeper penetration into the sample. The resolution of SEM depends on several factors, including the energy of the electron beam, the size of the electron spot, and the distance between the sample and the detector. The typical resolution of SEM is in the range of a few nanometers to tens of nanometers.


The images produced by SEM can provide information about the surface morphology, topography, and composition of the sample. In addition to imaging, SEM can also be used for elemental analysis, using energy-dispersive X-ray spectroscopy (EDS) or wavelength-dispersive X-ray spectroscopy (WDS). EDS and WDS are techniques used to determine the elemental composition of a sample, by measuring the X-rays emitted when the sample is bombarded with a high-energy electron beam.

 

Transmission Electron Microscopy (TEM)

Transmission electron microscopy (TEM) is a powerful technique for imaging the microstructure of materials at high resolution. TEM works by transmitting a focused electron beam through a thin sample, and detecting the electrons that pass through the sample. The electrons that pass through the sample are focused onto a fluorescent screen or a detector, which produces an image.


TEM operates under high vacuum conditions, and the samples need to be thin enough to allow the electrons to pass through. Sample preparation for TEM requires thinning the sample to a thickness of a few nanometers, using techniques such as ion milling, chemical etching, or mechanical polishing. TEM can provide high-resolution images of the microstructure of a wide range of materials, including metals, ceramics, polymers, and biological materials.


The resolution of TEM is determined by the wavelength of the electrons used in the beam. The wavelength of electrons is much shorter than that of visible light, allowing for much higher resolution imaging. The typical resolution of TEM is in the range of a few tenths of a nanometer to a few nanometers.


The images produced by TEM can provide information about the microstructure, crystal structure, and defects in the sample. In addition to imaging, TEM can also be used for elemental analysis, using energy-dispersive X-ray spectroscopy (EDS) or electron energy-loss spectroscopy (EELS). EDS and EELS are techniques used to determine the elemental composition of a sample, by measuring the X-rays or electrons emitted when the sample is bombarded with a high-energy electron beam.

 

Field-emission Scanning Electron Microscopy (FESEM)

Field-emission scanning electron microscopy (FESEM) is a type of SEM that provides higher resolution imaging than conventional SEM. FESEM works by focusing an electron beam onto a small area of the sample surface, and detecting the secondary electrons emitted from the sample. The secondary electrons are detected by a detector, which converts the signal into an image. FESEM operates under high vacuum conditions, and the samples need to be conductive or coated with a conductive material, such as gold or carbon.

FESEM uses a field-emission electron source, which produces a high-intensity electron beam with a very small electron spot size. This allows for higher resolution imaging than conventional SEM. The typical resolution of FESEM is in the range of a few nanometers to tens of nanometers.


The images produced by FESEM can provide information about the surface morphology, topography, and composition of the sample. In addition to imaging, FESEM can also be used for elemental analysis, using energy-dispersive X-ray spectroscopy (EDS) or wavelength-dispersive X-ray spectroscopy (WDS), similar to SEM.

 

Differences between SEM, TEM, and FESEM

While SEM, TEM, and FESEM share some similarities, they differ in their principles of operation, resolution, and sample preparation requirements. SEM provides high-resolution images of the surface of a wide range of materials, while TEM provides high-resolution images of the microstructure of materials. FESEM provides higher resolution imaging than conventional SEM, but still focuses on surface imaging rather than microstructure imaging. The resolution of SEM and FESEM is typically in the range of a few nanometers to tens of nanometers, while TEM provides higher resolution imaging in the range of a few tenths of a nanometer to a few nanometers.

Sample preparation requirements also differ between the techniques. SEM and FESEM require conductive or coated samples, while TEM requires thin samples that allow electrons to pass through. Sample preparation for SEM and FESEM is generally less time-consuming than TEM, which requires more extensive sample preparation, such as thinning the sample to a thickness of a few nanometers.

 

Applications of SEM, TEM, and FESEM

The applications of SEM, TEM, and FESEM are widespread and diverse, spanning a wide range of fields, including materials science, biology, chemistry, and physics. SEM is commonly used for surface imaging of materials, including metals, ceramics, polymers, and biological materials. It is also used for elemental analysis, using EDS or WDS. SEM is used in a variety of fields, including metallurgy, semiconductor industry, and forensic science.


TEM is used for high-resolution imaging of the microstructure of materials, including metals, ceramics, polymers, and biological materials. It is also used for elemental analysis, using EDS or EELS. TEM is widely used in materials science, condensed matter physics, and biology.


FESEM is used for high-resolution surface imaging of materials, including metals, ceramics, polymers, and biological materials. It is also used for elemental analysis, using EDS or WDS. FESEM is commonly used in materials science, semiconductor industry, and nanotechnology.

 

Conclusion

In conclusion, SEM, TEM, and FESEM are powerful techniques for imaging the microstructure and morphology of materials. Each technique has its own advantages and limitations, and the choice of technique depends on the specific application and the information required. With the advancement of technology, these techniques continue to evolve, providing even higher resolution imaging and more advanced analytical capabilities.

 

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