The consumables for electron microscopy

Electron microscopy is an important scientific tool that uses an electron beam instead of light to illuminate samples and generates high-resolution micrographs by collecting signals produced from the interaction between the electron beam and the sample. The consumables for electron microscopy include the following components:

1.      Electron microscope grids: Grids are thin films made of metal or carbon materials that support and hold the samples. They come in various shapes and aperture sizes such as square, circular, or elliptical holes. Before observing samples with an electron microscope, the samples are placed on the grids.

2.     Grid holders: Grid holders are devices made of metal that securely hold and carry the grids. They allow the grids to be placed on the specimen stage of the electron microscope, ensuring stable and accurate positioning of the samples inside the microscope.

3.     Carbon films: Carbon films are commonly used materials for sample preparation in electron microscopy. They can be used to fix and support biological samples or other types of samples. Carbon films offer low background noise and good electron transmittance, making them suitable for high-resolution electron microscopy.

4.     Metal films: Metal films are another common material used for sample preparation in electron microscopy. They are typically made of materials such as gold, platinum, or copper and can be used for observing inorganic samples or samples that require high conductivity.

5.     Conductive adhesive: Conductive adhesive is a type of adhesive used to fix the samples onto the grids. It provides electron conduction between the sample and the grid, reducing the accumulation of surface charges and interference.

Here is a simple guide for using an electron microscope:

1.      Prepare the sample: Select an appropriate sample and perform any necessary pre-processing steps. For biological samples, this might involve fixation, sectioning, staining, etc.

2.     Prepare the sample grid: Place the sample on the appropriate grid and use conductive adhesive to secure the sample onto the grid.

3.     Install the grid holder: Mount the grid holder with the sample onto the specimen stage of the electron microscope.

4.     Set microscope parameters: Adjust the acceleration voltage, focus, magnification, and other parameters of the electron microscope according to your requirements.

5.     Observe the sample: Insert the grid holder into the electron microscope and control the electron beam illumination and signal collection to observe the sample and obtain micrographs.

Please note that electron microscopy is a highly precise instrument, and it is important to strictly follow safety regulations and usage guidelines. The specific procedures may vary depending on the electron microscope model and manufacturer. It is recommended to carefully read the instructions manual and receive professional training before using the equipment.